【文獻(xiàn)標(biāo)題】Study on antitumor molecular mechanism of Alisols based on p53DNA

【作者】Fei Xu，Cai Lu， Qinan Wu，et.al

【作者單位】南京中醫(yī)藥大學(xué)（Nanjing University of Chinese Medicine）

【文獻(xiàn)中引用產(chǎn)品】

試劑-臺盼藍(lán)/臺盼蘭

【關(guān)鍵詞】Alisols，Antitumor activity，Molecular mechanism

【DOI】https://doi.org/10.1016/j.ijbiomac.2018.05.158

【影響因子(IF)】6.90

【出版期刊】《International Journal of Biological Macromolecules》

【產(chǎn)品原文引用】

Reagents: DMEM medium ａnd phosphate buffer saline (PBS)

(HyClone, USA); penicillin ａnd fetal bovine serum (FBS) (Gibco, USA);Methyl thiazolyl tetrazolium (MTT) (Beyotime, China); Dimethyl sulfoxide (DMSO) ａnd trypsin (Amresco, USA); Trypan-blue (Hengyuan Biological Technology, China); Paclitaxel standard (Sigma-Aldrich, USA);human breast cancer cell line (MCF-7) (ASTRI Cell Resource Center,China); Single-stranded DNA (Sangon Biotech, China):P1 promoter region of p53 gene (ss1: 5′-CCTCCTCCCCAACTCC-3′, ss2: 3′-GGAGGAGGGGT TGAGG-5′); Alisol A ａnd alisol B (Mansite Biotechnology,China) (structures shown in Fig. 1 [3]); Ethidium bromide (EB)(Macklin, China); Acetonitrile (LC, Merck, USA); Trihydroxymethyl aminomethane (Tris, BIO LINK, USA); Tris ａnd hydrochloric acid (HCl)were used to prepare Tris-HCl buffer (0.50 mol·L−1), pH 7.4; All other chemicals used were of analytical grade ａnd experimental water for ultrapure water.

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